loading
| Accessories for pT7Blue-2 Perfectly Blunt® Cloning Kit |
|
Product information
|
|
Avoid freeze/thaw
|
Yes
|
|
Components
|
| 40 rxn
| | • | 4 x 0.5 µg | Blunt Vector
| | • | 10 µl | Positive Control Insert
| | • | 2 x 100 µl | End Conversion Mix
| | • | 2 x 100 U | T4 DNA Ligase
| | • | 1.5 ml | Nuclease-free Water
| | • | 44 x 50 µl | NovaBlue Singles Competent Cells
| | • | 7 x 2 ml | SOC Medium
| | • | 10 µl | Test Plasmid |
|
|
Features and benefits
|
- T7 promoter upstream of 5′-UTR plus optimal eukaryotic translation initiation site for efficient transcription/translation analysis with the Single Tube Protein® System 3, T7
- lac promoter-driven expression in E. coli; compatible with EcoPro™ coupled transcription/translation system
- Blue/white screening of recombinants (requires that in-frame inserts contain a translation termination codon)
- N-terminal 15-aa S•Tag™ fusion for quantitative detection and purification
- Available as a Perfectly Blunt® Vector Kit containing linearized, dephosphorylated pT7Blue-2 for convenient, efficient cloning of PCR products
- SP6 promoter adjacent to EcoR I cloning site enables in vitro synthesis of RNA with minimal extraneous sequence
- Enterokinase cleavage site enables removal of all vector-encoded fusion sequences
- Extensive multiple cloning region
- High-copy replicon for high plasmid yields and phage f1 origin for single-stranded DNA production
|
|
Store and ship information
|
|
|
|
Storage
|
≤ -70°C
|
|
Ship
|
Dry Ice Only
Multiple Toxicity Values, refer to MSDS
|
