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70187  pT7Blue-3 Blunt Vector (linearized)

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

loading
 
Product number Qty/Pk Quantity Price
70187-3  20 rxn  loading
70187-4  40 rxn  loading
Prices are subject to change without notice.
The pT7Blue-3 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, a T7 promoter and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned at each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.

For enhanced convenience, please consider the Introductory pT7Blue-3 Perfectly Blunt®Cloning Kit (Cat. No. 70075), or the pT7Blue-3 Perfectly Blunt®Cloning Kit (Cat. No. 70182).


Product information
Avoid freeze/thaw Yes
Components
20 rxn40 rxn
2 × 0.5 µg4 × 0.5 µgBlunt Vector
10 µl10 µlPositive Control Insert
100 µl2 × 100 µlEnd Conversion Mix
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3′-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.

Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling

© Merck KGaA, Darmstadt, Germany, 2012


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