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Merck KGaA
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01 June 2012
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A fluorometric assay kit for the measurement of Protein Tyrosine Phosphatase (PTP) activity. The assay uses a fluorogenic phosphopeptide substrate quenched internally by resonance energy transfer. The substrate contains a fluorophore (MCA) and a quencher group (DNP) as well as a phosphotyrosine residue located between these groups. Reaction of the substrate with PTP followed by treatment of the dephosphorylated substrate with chymotrypsin results in the cleavage of the peptide, which results in an increase in fluorescence.
| Product information | |||
|---|---|---|---|
| Format | 96-well format | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Sensitivity | 0.2-0.4 pmol | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | PTP Substrate, Assay Buffer, Chymotrypsin Solution, Control Peptide, Microtiter Plate, Stop Solution, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 23-24-26-36/37 Do not breathe fumes. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. |
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| R Phrase |
R: 36/37/38-42 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation. |
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