70826  pTriEx™-2 DNA

The pTriEx™-2 vector¹ is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-2 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λp_L and λlp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site. Alternatively, native protein can be generated by cloning into the PshA I or Sma I sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively.
¹patent pending
Note- this product is not available in Japan