555468
Rho-GDIα, Human, Recombinant, E. coli
Rho GDP Dissociation Inhibitor
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Recombinant, human Rho-GDIα fused to GST and expressed in E. coli. Expressed as a GST fusion protein with the GST later removed by thrombin cleavage. Suitable for use in in vitro Rho GTPase activity assays. Rho-GDI binds to the GTPase Rho in its GDP bound form and prevents it from being converted to the active, GTP-bound form by the guanine nucleotide exchange factor.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 25 mM Tris-HCl, 2.5 mM MgCl2, 1 mM CaCl2, 30% glycerol, pH 8.0. | ||
| Molar mass | 30000 | ||
| Avoid freeze/thaw | Yes | ||
| Biological activity | Significantly inhibits Rho A GEF exchange activity at a concentration of ~2 µM | ||
| Purity | ≥90% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Carcinogenic / Teratogenic |
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