71250
RingMaster™ Nuclease
Eliminates linear DNA from plasmid preparations
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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RingMaster™ Nuclease is a recombinant exonuclease that hydrolyses linear double-stranded DNA from a 5′-phosphorylated end, releasing 5′-phosphomononucleotides. The enzyme does not affect circular supercoiled DNA.
Bacterial genomic DNA often contaminates standard plasmid preparations, especially in the case of low-copy number plasmids. Using RingMaster Nuclease to eliminate linear DNA as the final treatment step in your plasmid purification protocol improves plasmid DNA quality. One unit of the enzyme is sufficient for removal of > 95% bacterial genomic DNA contamination from 1 µg plamid DNA in 1 hour at 37°C. The enzyme can be completely inactivated by heating treated plasmid DNA for 10 minutes at 75°C.
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| Unit definition | One unit is defined as the amount of enzyme that digests 200 ng of lambda DNA in 30 minutes at 37°C. | |||||||||||
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| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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