557400
RNA Polymerase II-CTD, Recombinant, E. coli
RNA Polymerase II, Carboxy-Terminal Domain
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01 June 2012
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Recombinant carboxy-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II expressed in E. coli. Contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser, which is highly conserved among eukaryotic organisms. The RNA pol II-CTD is essential for transcription, for organization of transcription foci within the nucleus, and for pre-mRNA processing, and is targeted by several kinases and phosphatases.
Note: 1 KU = 1000 units
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9. Supplied with a Dilution Buffer. | ||
| Molar mass | 44000 | ||
| Avoid freeze/thaw | Yes | ||
| Activity | 1 unit/ng protein | ||
| Biological activity | 20 units are sufficient for reconstituted transcription assays; 100 units are sufficient for protein-protein interaction assays. | ||
| Purity | ≥95% by SDS-PAGE | ||
| Contaminants | DNase, RNase, and protease activity: none detected | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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