For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
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Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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The Rosetta™ 5615 is a new
T7Select® Phage Display System host strain, closely related to the Rosetta host strain, available for recombinant protein expression. Rosetta host strains are Tuner™ derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. This new strain carries as ampicillin-resistant, medium–copy number plasmid, pAR5615, that expresses the native T7 capsid protein under the control of the lacUV5 promoter.
Genotype
F– ompT hsdSB(rB– mB–) gal dcm lacY1 pRARE22 (CmR) pAR5615 (ApR)
Genotype
F– ompT hsdSB(rB– mB–) gal dcm lacY1 pRARE22 (CmR) pAR5615 (ApR)
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| Declaration | Rosetta and Tuner are trademarks of EMD Biosciences, Inc., Madison, WI USA | ||
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| Storage | ≤ -70°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
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