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01 junio 2012
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Rosetta-gami™ B 5615 is a new
T7Select® Phage Display System host strain, closely related to the Rosetta-gami host strain, available for recombinant protein expression. Rosetta-gami host strains are Origami™ derivatives that combine the enhanced disulfide bond formation resulting from trxB/gor mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. This new strain carries as ampicillin-resistant, medium–copy number plasmid, pAR5615, that expresses the native T7 capsid protein under the control of the lacUV5 promoter.
Genotype
F– ompT hsdSB(rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pRARE2 (CmR) pAR5615 (ApR)
Genotype
F– ompT hsdSB(rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pRARE2 (CmR) pAR5615 (ApR)
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| Categoría de alamacenamiento | ≤ -70°C | ||
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Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
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