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The T7•Tag® Antibody is a mouse monoclonal antibody (IgG
2b) directed against the 11 amino acid gene
10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with
biotin and as a direct conjugate with
alkaline phosphatase or
horseradish peroxidase.
| Specificity | T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal |
| Species/Isotype | Mouse monoclonal IgG2b |
| Cross-reactivity | Negligible with bacterial, insect or mammalian cell lysates |
| Sensitivity | 1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3) |
| Form | Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein |
| Working dilution | 1:5,000–1:10,000 |
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 50 µg | (sufficient for 50 Western blots (10 cm × 10 cm) or 50 immunoprecipitation assays)
| | or 250 µg | (sufficient for 250 Western blots or 250 immunoprecipitation assays)
| | • | | Positive Control Extract containing 31.1 kDa target protein |
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Store and ship information
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Storage
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-20°C
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Ship
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Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS
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