574900
TBP, His•Tag®, Human, Recombinant, E. coli
TATA Box Binding Protein
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Recombinant, human TBP fused to a His•Tag® sequence and expressed in E. coli. The TATA-binding protein (TBP) is believed to function as an essential factor of general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor-suppressor proteins. Supplied with dilution buffer.
Note: 1 KU = 1000 units.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM KCl, 20 mM Tris-HCI, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9. | ||
| Molar mass | 38000 | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is sufficient for a gel mobility assay in a 20 µl reaction; 10 units are sufficient for a 25 µl reconstituted transcription reaction; and 100 units are sufficient for a protein-protein interaction assay detected by immunoblot system. Note: 1 unit = 1 ng. | ||
| Activity | 1 unit/ng protein | ||
| Purity | ≥95% by SDS-PAGE | ||
| Contaminants | DNase, RNase, and protease activity: none detected | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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