539718
TruLight™ Phosphoinositide 3-Kinase Assay Kit
TruLight™ PI3K Assay Kit
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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A sensitive and high speed homogeneous assay for the detection of enzyme activity and for screening of Phosphoinositide 3-Kinase (PI3K) inhibitors in a high-throughput screening (HTS) format. This kit is based on a fluorescence superquenching technology that does not require antibodies or radioactive label.
| Substitutes | |
|---|---|
| CBA101 | PI 3-K Assay Kit |
| Substitutes for TruLight™ Phosphoinositide 3-Kinase Assay Kit | |
| CBA101 | PI 3-K Assay Kit |
| Product information | |||
|---|---|---|---|
| Format | 384-well plate | ||
| Form | 384 Tests | ||
| Detection method | Fluorescence | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | Assay Buffer, DTT, 10X Sensor, Sensor Dilution Buffer 2, and a user protocol. | ||
| Declaration | Sold under license of U.S. Patent 6,589,731. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Quencher-labeled substrate (represented by the forked lines) is phosphorylated by PI 3-K and binds to the Sensor (yellow circle) via the phosphate-metal ion interaction. The fluorescence is quenched (gray circle) as a result of this interaction (i.e., turn-off mode). The assay can also be read in turn-on mode by measuring the emission of the energy transferred from Sensor to fluorescent dye.  The activity of PI 3-Kβ was measured using the various wavelength modes as outlined in the Detailed Protocol. |
