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WideScreen® Activity Assays

Activity assays are an effective and efficient way to determine the activity of your particular enzyme, the efficiency of your inhibitors or activators, or the results of upstream antagonism.  The activity assays provided by EMD are offered in a convenient plate-based set-up that allows for either manual or higher throughput analysis.
K-LISA are a subset of assays particularly tailored for the measurement of kinases from either recombinant or lysates sources.  The assay kits measure the kinase activity by utilizing a technique in which phosphorylation of a target is detected using phosphospecific antibodies of the phosphorylated protein substrates of the kinase of interest
A large number of caspases are also offered.  These assays work on the principle that caspases cleave signature peptide sequence of their targets.  When the target is cleaved, either a Fluorimetric or colorimetric substrate is released.  This allows for an easy, quantifiable detection of the caspase activity. 

When to use

  • To measure enzymatic (e.g. kinase, caspase, etc.) activity and determine the effects of activators or inhibitors.
  • The kits vary in sample compatibility, so this should be considered carefully when choosing an activity assay kit.

Advantages

  • Direct measurement of:
    • Enzymatic activity
    • Effects of inhibitors/activators

Sample Type

  • Varies – cell lysates, partially purified or purified enzyme, tissue homogenates, cell extracts

Detection

  • Colorimetric
  • Western blot

Equipment Required

  • Colorimetric ELISA plate reader
  • Western blotting equipment

 Products

Producto Formato Formulario Sensibilidad Rango de ensayo Tiempo de ensayo Tipo de muestra Núm. prod.
20S Proteasome Assay Kit, SDS-Activated Cuvette or 96-well plate       30 min   539158
Active Caspase-8 Assay Kit 96-well plate 182 Tests       Cells CBA046
Active Caspase-9 Assay Kit 96-well plate 480 Tests       Cells CBA047
Akt Activity Immunoassay Kit Immunoblot 40 Tests     4 h Cell lysates, tissue extracts 124007
ATP Assay Kit 96-well, luminometer cuvette , or Beta counter 200 Tests     1 h Blood, milk, urine, soil, and sludge 119107
Calcineurin Assay Kit, Colorimetric 96-well plate 96 Tests     3.5 h Purified human calcineurin 207005
Calcineurin Cellular Activity Assay Kit, Colorimetric 96-well plate 96 Tests     7 h Tissue/cellular extracts 207007
Calpain Activity Assay Kit, Fluorogenic 96-well plate 96 Tests     1.5 h Cell lysates, plasma, serum QIA120
cAMP Direct Immunoassay Kit, Colorimetric 96-well format 100 Tests 390 fmol/ml (non-acetylated), 37 fmol/ml (acetylated) 0.78 - 200 pmol/ml (non-acetylated), 0.078-20 pmol/ml (acetylated) 4.5 h Serum, cell, and tissue samples 116811
Caspase-10 Assay Kit, Fluorometric 96-well plate 96 Tests     2-3 h Cell lysates 218811
Caspase-1 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests     2.5-3 h Cell lysates 218790
Caspase-1 Assay Kit, Fluorometric 96-well plate 100 Tests     3-3.5 h cell lysates 218791
Caspase-1 Inhibitor Screening Assay Kit 96-well plate 96 Tests     2.5-3 h Purified protein 218734
Caspase-2 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests     2.5-3 h Cell lysates 218792
Caspase-2 Assay Kit, Fluorometric 96-well plate 96 Tests     2.5-3 h Cell lysates 218793
Caspase-3 Activity Assay Kit, Fluorometric 96-well plate 96 Tests     2.5-3 h Cell lysates QIA70
Caspase-3 Activity Assay Kit, HTS 96-well plate 100 or 500 Tests     3 h Cultured cells or purified protein HTS02
Caspase-3 Cellular Activity Assay Kit 96-well plate 96 Tests     2.5-3 h Cell extracts 235419
Caspase-3 Detection Kit (FITC-DEVD-FMK) Flow cytometry, fluorescence microscopy 100 Tests     1.5 h Cultured cells QIA91
Caspase-3 Detection Kit (Red-DEVD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Cultured cells QIA93
Caspase-3 Immunoassay/Activity Kit, Fluorometric 96-well plate 96 Tests     6 h Cultured cells QIA107
Caspase-3 Inhibitor Screening Assay Kit 96-well plate 96 Tests     2 h Recombinant or purified caspase-3 235418
Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux® G2D2) Flow cytometry or fluorescence microscopy 30 Tests     1.5-2 h Intact cells 235432
Caspase-3 Intracellular Activity Assay Kit I (PhiPhiLux® G1D2) Flow cytometry or fluorescence microscopy 30 Tests     2 h Intact cells 235430
Caspase-5 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests       Cell lysate 218804
Caspase-6 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests     1.5-2.5 h Cell lysate 218802
Caspase-7 Immunoassay/Activity Kit, Fluorometric 96-well plate 96 Tests     6 h Cultured cells QIA108
Caspase-8 Activity Assay Kit, Fluorometric 96-well plate 96 Tests     2-3 h Cell lysate QIA71
Caspase-8 Assay Kit 96-well plate 96 Tests     2.5-3.5 h Purified caspase-8 218770
Caspase-8 Detection Kit (FITC-IETD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Intact cells QIA113
Caspase-8 Detection Kit (Red-IETD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Intact cells QIA114
Caspase-9 Activity Assay, HTS 96-well plate 96 Tests     3 h Purified protein, cell lysate, or cultured cells HTS04
Caspase-9 Activity Assay Kit, Fluorometric 96-well plate 96 Tests     2-3 h Cell lysate QIA72
Caspase-9 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests     2-3 h Cell lysate 218824
Caspase-9 Detection Kit (FITC-LEHD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Intact cells QIA115
Caspase-9 Detection Kit (Red-LEHD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Intact cells QIA116
Caspase Detection Kit (FITC-VAD-FMK) Flow Cytometry or Fluorimeter 100 Tests     1.5 h Intact cells QIA90
Caspase Detection Kit (Red-VAD-FMK) Flow cytometry or fluorescence microscopy 100 Tests     1.5 h Intact cells QIA92
Catalase Assay Kit 96-well plate 96 Tests   0.25-4 nmol/min/ml 2 h Plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates 219265
Cathepsin B Detection Kit Fluorescence microscopy       4-6 h Cells 219346
Cathepsin K Detection Kit Fluorescence microscopy       4-6 h Cells 219347
Glutathione Assay Kit Cuvette 100 Tests 5.0 µM RSH   1.5 h Cell lysates, tissue homogenates 354102
Glutathione Assay Kit II 96-well plate 96 Tests   0 - 16 µM for GSH or 0 - 8 µM for GSSG 3 h Plasma, serum, erythrocyte lysate, tissue samples, and cultured cells 354103
Glutathione Peroxidase Assay Kit 96-well plate 96 Tests   50-344 nmol/min/ml 2 h Cells, tissues, plasma 353919
Glutathione Peroxidase, Cellular Assay Kit Cuvette 100 Tests   5.6-24 mU/ml 2 h Cell lysates, tissue extracts, erythrocyte lysates 354104
Glutathione Reductase Assay Kit II 96-well plate 96 Tests   20-255 nmol/min/ml 2 h Plasma, erythrocyte lysates, tissue homogenates, and cell lysates 359963
GSH/GSSG Ratio Assay Kit Cuvette 200 Tests 9 nM GSSG   1.5 h cell lysates, tissue extracts, blood 371757
InnoZyme™ Cathepsin B Activity Assay Kit, Fluorogenic 96-well plate 96 Tests 0.63 ng/ml 0.09 - 100 ng/ml 30 min Cells and tissues CBA001
InnoZyme™ Cathepsin D Immunocapture Activity Assay Kit, Fluorogenic 96-well plate 96 Tests 0.2 ng/ml 3.13-50 ng/ml 1.5 h Biological fluids and culture supernatant CBA002
InnoZyme™ Cathepsin L Activity Kit, Fluorogenic 96-well plate 96 Tests 1.2 ng/ml 1.56-100 ng/ml 1.5 - 2 h Cell lysates, tissues, or purified cathepsin L CBA023
InnoZyme™ Cathepsin S Activity Assay Kit 96-well plate 96 Tests   1-40 ng/ml 2.5 h Cells, tissues, purified enzyme CBA050
InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay kit, Fluorogenic 96-well plate 96 Tests 20 ng/ml   2 - 6 h Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme CBA003
InnoZyme™ Granzyme B Immunocapture Activity Assay Kit 96-well plate 96 Tests 0.01 ng/ml (5 h incubation) and 0.3 pg/ml (24 h incubation) 0.0625- 2 ng/ml (5 h incubation) and 3.125 - 50 pg/ml (24 h incubation) 7 - 30 h Cell lysates, tissue extracts, and body fluids CBA014
InnoZyme™ Human Neutrophil Elastase Immunocapture Activity Assay Kit 96-well plate 96 Tests   0.625-20 ng/ml (4 h) and 62.5-2000 pg/ml (24 h) 6 h Cell lysates, body fluids, for screening HNE inhibitors CBA016
InnoZyme™ Myeloperoxidase Activity Kit 96-well plate 96 Tests 330 pg/ml 5 -100 ng/ml 2 h Cell lysates, CSF, and serum CBA024
InnoZyme™ TACE Activity Kit 96-well plate 96 Tests   5-100 ng/ml as measured with purified recombinant human TACE   Cells CBA042
JNK Activity Immunoassay Kit Immunoblot 40 Tests       Cell lysates 420115
K-LISA™ Akt Activity Kit 96-well plate 96 Tests 0.05 mUnits 6-100 mU/well 3 h Cell lysates, tissue extracts, purified enzyme CBA019
K-LISA™ Checkpoint Activity Kit 96-well plate 96 Tests 25 pg (0.018 mU) purified Chk1 25-725 µU (Chk1) and 260 µU - 26 mU (Chk2) 3 h Cell lysates, tissue extracts, purified enzymes CBA020
K-LISA™ IKKβ Inhibitor Screening Kit 96-well plate 96 Tests   5-10 ng   Purified enzyme CBA044
K-LISA™ mTOR Activity Kit 96-well plate 96 Tests       Purified or partially purified enzyme CBA055
K-LISA™ mTOR (Recombinant) Activity Kit 96-well plate 96 Tests         CBA104
K-LISA™ PTK E4Y Reaction Plate   96-well plate         539702
K-LISA™ PTK EAY Reaction Plate   96-well plate         539703
K-LISA™ PTK EY Reaction Plate   96-well plate         539704
K-LISA™ PTK Screening Kit 96-well plate 216 Tests, 72 Controls     3 h Cell lysates, tissue extracts, purified enzyme 539701
LCAT Activity Assay Kit, Fluorometric   100 Tests     4.5 - 8.5 h Plasma 428900
Nitric Oxide Assay Kit, Colorimetric 96-well plate 80 Tests     1-1.5 h Aqueous solutions 482650
Nitric Oxide Assay Kit, Fluorometric 96-well plate 192-288 Tests     3-5 h Aqueous solutions 482655
Nitric Oxide Synthase Assay Kit Scintillation vial 50 Tests picomolar range 2 h   Tissue or cell extracts 482700
Nitric Oxide Synthase Assay Kit, Colorimetric 96-well plate 96 Tests     2 h Plasma, serum, urine, tissue culture medium, and tissue homogenates 482702
OxyDNA Assay Kit, Fluorometric   50 Tests     4 h Cells 500095
Protease Assay Kit Cuvette or 96-well plate 200 Tests 10 ng/ml   3 h Biological samples 539125
Protein Kinase Assay Kit, Non-Radioactive 96-well plate 96 Tests       Cell lysates, purified enzymes 539484
PTP1B Assay Kit, Colorimetric 96-well plate 96 Tests     2 h Purified enzyme 539736
PTP Assay Kit, Fluorometric 96-well format 96 Tests 0.2-0.4 pmol   2 h Purified enzyme 539742
β-Secretase Activity Assay Kit, Fluorogenic Cuvette or 96-well plate 100 Tests     1.5 h Cell lysates, tissue extracts, purified BACE 565785
Superoxide Anion Detection Kit, Chemiluminescent Round-bottom polystyrene luminometer tubes 100 Tests     1.5 h Cell cultures 574590
Superoxide Dismutase Assay Kit II 96-well plate 96 Tests   0.025-0.25 units per ml SOD 1.5 h Plasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates 574601
Total Antioxidant Status Assay Kit Cuvette 50 Tests   0-25 nmol/10 µl plasma   Serum, plasma, tissue extracts, plant extracts, beverages 615700
Urokinase Activity Assay Kit, Fluorogenic 96-well plate 100 Tests 0.01 U/ml 0.78 - 50 U/ml 1.5 h Cell lysates, plasma, and serum, for screening uPA inhibitors QIA125

© Merck KGaA, Darmstadt, Alemania, 2012


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