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115943  Methylene blue (C.I.52015)
for microscopy Certistain®

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64293 Darmstadt
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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17 April 2014


Methylene blue is used in microscopy in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method. Bacteria appear blue. It is also used in hematology: Methylene blue and eosin are the main components in staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements

Product number Packaging Qty/Pk Quantity Price
1159430025  Glass bottle   25 g  loading
1159430100  Glass bottle   100 g  loading
Prices are subject to change without notice.
115937 Fuchsin (C.I. 42510) for microscopy Certistain®
Alternative products for Methylene blue (C.I.52015)
Accessories for Methylene blue (C.I.52015)
115937 Fuchsin (C.I. 42510) for microscopy Certistain®
Product information
Synonyms Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
Hill Formula C16H18ClN3S * x H2O
Chemical formula C16H18ClN3S * x H2O (x=2-3)
HS Code 3204 13 00
EC number 200-515-2
Molar mass 319.86 g/mol (anhydrous)
Chemical and physical data
Solubility 50 g/l (20 °C)
Melting point 180 °C (decomposition)
Molar mass 319.86 g/mol (anhydrous)
Bulk density 400 - 600 kg/m3
pH value 3 (10 g/l, H2O, 20 °C)
Safety information according to GHS
Hazard Statement(s) H302: Harmful if swallowed.
Signal Word Warning
Hazard Pictogram(s)
Storage class 10 - 13 Other liquids and solids
WGK WGK 2 water endangering
Disposal 3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety information
R Phrase R 22
Harmful if swallowed.
Categories of danger harmful
Hazard Symbol
Toxicological data
LD 50 oral LD50 rat 1180 mg/kg
Methylene blue (C.I.52015), molecular structure

© Merck KGaA, Darmstadt, Germany, 2014


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