ELISAs
ELISAs (Enzyme-linked immunosorbent assays) are a simple, yet effective assay tool used in the measurement of either total or phosphorylated protein levels in biological samples such as cell lysates or serum. ELISAs are conveniently offered with capture antibody-coated 96-well plates. This binds the target of interest when the sample is added and the plate is washed. To give an added layer of specificity, a detection antibody is added to the wells. This antibody binds to a different epitope than the first, adding a dual layer of specificity to the assay. This allows the user to get a clearer, more quantifiable assay. The protein levels are quantitated using the standard HRP/TMB detection method and a standard plate reader that are readily found in most labs.
When to use
- Use to quantitate levels of total or phosphorylated proteins
Advantages
- Specific, sensitive detection of protein levels in non-purified samples
- Detection can be species-specific
- No special equipment required
Sample Type
- Variable. Often tissue homogenates, cell extracts, partially purified proteins, or serum. Refer to individual kits for more information.
Detection
- Colorimetric
Equipment Required
- Colorimetric ELISA plate reader
Interested in multiplexing? Check out our WideScreen® BeadPlex™ assays that allow for the simultaneous detection of many proteins in the same well. Save time, money, and sample.
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