Benzonase® endonuclease for DNA and RNA removal
In your manufacturing process, we understand that it’s critical that you eliminate DNA impurities. Removing DNA and RNA upstream can improve downstream processing later on – and the only effective biochemical method is enzymatic cleavage, using our Benzonase® endonuclease.
Benzonase® is a unique, genetically-engineered endonuclease that is not of animal origin, and is only available from Merck Millipore. Produced from E.coli, this non-specific, recombinant endonuclease cleaves all kinds of DNA and RNA variants into fragments that comprise <10 soluble base pairs. This leads to an utmost minimum of nucleic acid load, and hence to an acceptable lower viscosity of the process fluid.
Benzonase® has several advantages over conventional methods of removal:
- Highest purity
We manufacture Benzonase® endonuclease using state-of-the-art production techniques and standards. The result is a top quality product that enables the highest possible purity standards for your biopharmaceuticals.
- Wide range of applications
Benzonase® endonuclease has an exceptionally high level of specific activity for nucleic acids without any detectable proteolytic activity. It is effective in a wide range of operating conditions and applications, including the following:
- Reduced process time
Benzonase® endonuclease has proven its value in the laboratory, and in many processes throughout the vaccine, biopharmaceutical and biotechnological industry.
- Enhanced productivity
By ensuring higher purity, higher recovery and easier scale-up, Benzonase® endonuclease helps maximize the productivity of your entire production process.
• purification of vaccines, proteins and other biologicals
• reduction of viscosity caused by nucleic acids
• sample preparation in electrophoresis and chromatography.