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Benzonase® endonuclease for DNA and RNA removal

In your manufacturing process, we understand that it´s critical that you eliminate DNA impurities. Removing DNA and RNA can improve downstream processing later on — and the only effective biochemical method is enzymatic cleavage, using our Benzonase® endonuclease.

Benzonase® is a unique, genetically-engineered endonuclease that is only available from Merck Millipore. Produced in E.coli, this non-specific, recombinant endonuclease cleaves all kinds of DNA and RNA variants into fragments that comprise < 8 soluble base pairs. This leads to an utmost minimum of nucleic acid load, and hence to an acceptable lower viscosity of the process fluid.

Wide range of applications
Benzonase® endonuclease has an exceptionally high level of specific activity for nucleic acids without any detectable proteolytic activity. It is effective in a wide range of operating conditions and applications, including the following:

  • purification of vaccines, proteins and other biologicals
  • reduction of viscosity caused by nucleic acids
  • sample preparation in electrophoresis and chromatography
  • prevention of cell clumping

What’s in Benzonase® endonuclease for you?

  • the capability to attack and degrade all forms of DNA and RNA
  • the absence of detectable proteolytic activity
  • the assurance that you’ll meet industry requirements for reliable supply and consistent high quality thanks to GMP manufacturing
  • the support by our EMPROVE® bio dossier and a DMF type II file (FDA Reg. No. BBMF 5403)

To meet the widest possible range of processing and cost requirements, Benzonase® endonuclease is available in different package sizes and grades of purity:

Designation Package size Ord.No.

Benzonase® endonuclease, purity grade II (≥90%), for biotechnology

100,000 U/vial

1.01654.0001

Benzonase® endonuclease, purity grade II (≥90%), for biotechnology

500,000 U/vial

1.01656.0001

Benzonase® endonuclease, purity grade I (≥99%) suitable for biopharmaceutical production EMPROVE® bio

100,000 U/vial

1.01695.0001

Benzonase® endonuclease, purity grade I (≥99%) suitable for biopharmaceutical production EMPROVE® bio

500,000 U/vial

1.01697.0001

Benzonase® endonuclease, purity grade I (≥99%) suitable for biopharmaceutical production EMPROVE® bio

5,000,000 U/vial

1.01697.0010

Benzonase® endonuclease ELISA Kit II for the
immunological detection of Benzonase® endonuclease

5 plates
(8 x 12)
plus reagents

1.01681.0001

Working with Benzonase® endonuclease for the first time – or using it in a new application?
Here are answers to the most frequently asked questions about Benzonase® endonuclease.

For more information, please contact us.

© Merck KGaA, Darmstadt, Germany, 2014


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