Eshmuno® HCX - new high salt-tolerant multi-mode chromatography resin
Eshmuno® chromatography resins are a new and unique family of ion exchange resins that are designed specifically for highly productive downstream bioprocessing. The first members of the family, Eshmuno® S resin and Eshmuno® Q resin, are smart ion exchanger designed for fast and efficient purification of antibodies.
Eshmuno® HCX media, a perfect line extension to the existing Eshmuno® chromatography resin family, is the result of extensive investigation to address the developing needs of the industry of performing state-of-the-art chromatography steps in the manufacture of today’s approved biotherapeutics.
Eshmuno® HCX: outstanding performance at higher salt concentration
Eshmuno® HCX media, the newest member of the innovative Eshmuno® resin product offering, is a smart mixed-mode resin, that couples the renowned tentacle structure with the new hydrophilic polyvinyl ether base matrix. As a result, Eshmuno® HCX media offers outstanding performance results at higher salt concentration in typical ion exchange and flow-through applications.
|Superior capacities over a wide range of process conditions|
|Both, static and dynamic testing methods demonstrate the wide range of operating conditions of Eshmuno® HCX media achieving high protein binding capacities at high salt levels. The open interconnected pore structure maintains rapid mass transfer, resulting in these higher dynamic capacities being achievable over a wide range of process conditions.|
|Eshmuno® HCX: media characteristics|
|Type of chromatography
||Multi-modal cation exchange chromatography
||Sulfo carboxy and phenyl groups
||Surface grafted rigid polyvinyl ether hydrophilic polymer
|Mean particle size (d50)
||75 - 95 μm
|IgG Dynamic Capacity
(pH 5.5, 5 min residence time, 100% breakthrough)
|≥ 50 mg/ml|
|Linear flow rate
||up to 1000 cm/h < 2.5 bar net pressure
||pH 2 up to 12
Eshmuno® HCX: rigid base beads for easy packing
The highly cross-linked hydrophilic polyvinyl derivative assures outstanding robustness compared to other bead-based technologies.
The spherical regular shape of the beads and its appropriate particle size distribution allows for easy packing and scale-up. Its open and regular pore system enables a good accessibility of the proteins to the ligand. The tentacles, forming a three-dimensional ion exchange network, are also supporting this superior accessibility.