AP1064
PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer300 Rabbit pAb
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
02 iunie 2012
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Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser300 phosphorylation site of human PDH-E1α | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Da | ||
| Positive control | HEK293 cells | ||
| Negative control | HEK293 cells treated with dichloroacetate | ||
| Informatii despre stocare si transport | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
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 Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.  Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains. |
