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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
02 iunie 2012
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This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
| Product information | |||
|---|---|---|---|
| Format | Cell extraction | ||
| Form | 100 Extractions | ||
| Avoid freeze/thaw | Da | ||
| Sample type | Cells | ||
| Kit contains | Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol. | ||
| Informatii despre stocare si transport | |||
|---|---|---|---|
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 23-26-36/37/39-45 Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 23/24/25-32-34-36/37/38-41 Toxic by inhalation, in contact with skin and if swallowed. Contact with acids liberates very toxic gas. Causes burns. Irritating to eyes, respiratory system and skin. Risk of serious damage to eyes. |
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| Data | |||
|---|---|---|---|
 Mitochondrial and cytosolic fractions were isolated from HEPG2 cells using QIA88 ProteoExtract® Cytosol/ Mitochondria Fractionation Kit. Mitochondrial pellets were resuspended in PBS. The protein concentration of each fraction was determined using the BCA assay. DTT was omitted from the Cytosol Extraction Buffer as it can interfere with concentration determination in the BCA assay. Equivalent amounts (10 µg) of HEPG2 homogenate (total protein), cytosolic, and mitochondrial fractions were analyzed by Western blot for proteins localized to different cellular regions as indicated. Representative results from 2 independent fractionations are shown. |
