71975 KOD Xtreme™ Hot Start DNA Polymerase

KOD system optimized for difficult targets

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Телефон: +49 6151 72-0
Факс: +49 6151 72 2000

02 Июнь 2012

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Номер продукта Qty/Pk Количество Цена

71975-3

Номер продукта	Qty/Pk	Количество	Цена
71975-3	200 u	Обновить	загрузка
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200 u

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Prices are subject to change without notice.

The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt^® and LIC Vector Kits.

*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Информация о продукте.

Unit definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), and 150 µg/ml activated calf thymus DNA.

Components

•1 × 200 U KOD Xtreme™ Hot Start DNA Polymerase

•3 × 1.7 ml 2X Xtreme Buffer

•2 × 1 ml dNTPs (2 mM each)

Features and benefits

Optimized for the highest PCR success rate, even with the most difficult targets
10X higher fidelity than Taq blends
Efficiently amplifies up to 90% GC-content DNA templates
Amplifies genomic targets up to 24 kbp
Amplifies phage/Lambda targets up to 40 kbp
Eliminates mispriming and primer-dimer formation
Convenient ambient-temperature setup compatible with automation
See Figures at the bottom of this page for addtitional product information

Информацию о хранении и способе достаки

Storage	-20°C
Ship	Shipped with Blue Ice or with Dry Ice Irritant

Информация по технике безопасности
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.