WideScreen® Activity Assays
Activity assays are an effective and efficient way to determine the activity of your particular enzyme, the efficiency of your inhibitors or activators, or the results of upstream antagonism. The activity assays provided by EMD are offered in a convenient plate-based set-up that allows for either manual or higher throughput analysis.
K-LISA are a subset of assays particularly tailored for the measurement of kinases from either recombinant or lysates sources. The assay kits measure the kinase activity by utilizing a technique in which phosphorylation of a target is detected using phosphospecific antibodies of the phosphorylated protein substrates of the kinase of interest
A large number of caspases are also offered. These assays work on the principle that caspases cleave signature peptide sequence of their targets. When the target is cleaved, either a Fluorimetric or colorimetric substrate is released. This allows for an easy, quantifiable detection of the caspase activity.
When to use
- To measure enzymatic (e.g. kinase, caspase, etc.) activity and determine the effects of activators or inhibitors.
- The kits vary in sample compatibility, so this should be considered carefully when choosing an activity assay kit.
Advantages
- Direct measurement of:
- Enzymatic activity
- Effects of inhibitors/activators
Sample Type
- Varies – cell lysates, partially purified or purified enzyme, tissue homogenates, cell extracts
Detection
- Colorimetric
- Western blot
Equipment Required
- Colorimetric ELISA plate reader
- Western blotting equipment
Products
| Product | Format | Form | Sensitivity | Assay Range | Assay Time | Sample Type | Cat. No. |
|---|---|---|---|---|---|---|---|
| 20S Proteasome Assay Kit, SDS-Activated | Cuvette or 96-well plate | 30 min | 539158 | ||||
| Calpain Activity Assay Kit, Fluorogenic | 96-well plate | 96 Tests | 1.5 h | Cell lysates, plasma, serum | QIA120 | ||
| Caspase-3 Activity Assay Kit, Fluorometric | 96-well plate | 96 Tests | 2.5-3 h | Cell lysates | QIA70 | ||
| Caspase-3 Cellular Activity Assay Kit | 96-well plate | 96 Tests | 2.5-3 h | Cell extracts | 235419 | ||
| Caspase-3 Detection Kit (FITC-DEVD-FMK) | Flow cytometry, fluorescence microscopy | 100 Tests | 1.5 h | Cultured cells | QIA91 | ||
| Caspase-3 Detection Kit (Red-DEVD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Cultured cells | QIA93 | ||
| Caspase-3 Inhibitor Screening Assay Kit | 96-well plate | 96 Tests | 2 h | Recombinant or purified caspase-3 | 235418 | ||
| Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux® G2D2) | Flow cytometry or fluorescence microscopy | 30 Tests | 1.5-2 h | Intact cells | 235432 | ||
| Caspase-3 Intracellular Activity Assay Kit I (PhiPhiLux® G1D2) | Flow cytometry or fluorescence microscopy | 30 Tests | 2 h | Intact cells | 235430 | ||
| Caspase-7 Immunoassay/Activity Kit, Fluorometric | 96-well plate | 96 Tests | 6 h | Cultured cells | QIA108 | ||
| Caspase-8 Activity Assay Kit, Fluorometric | 96-well plate | 96 Tests | 2-3 h | Cell lysate | QIA71 | ||
| Caspase-8 Detection Kit (FITC-IETD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Intact cells | QIA113 | ||
| Caspase-8 Detection Kit (Red-IETD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Intact cells | QIA114 | ||
| Caspase-9 Activity Assay Kit, Fluorometric | 96-well plate | 96 Tests | 2-3 h | Cell lysate | QIA72 | ||
| Caspase-9 Detection Kit (FITC-LEHD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Intact cells | QIA115 | ||
| Caspase-9 Detection Kit (Red-LEHD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Intact cells | QIA116 | ||
| Caspase Detection Kit (FITC-VAD-FMK) | Flow Cytometry or Fluorimeter | 100 Tests | 1.5 h | Intact cells | QIA90 | ||
| Caspase Detection Kit (Red-VAD-FMK) | Flow cytometry or fluorescence microscopy | 100 Tests | 1.5 h | Intact cells | QIA92 | ||
| Glutathione Assay Kit | Cuvette | 100 Tests | 5.0 µM RSH | 1.5 h | Cell lysates, tissue homogenates | 354102 | |
| GSH/GSSG Ratio Assay Kit | Cuvette | 200 Tests | 9 nM GSSG | 1.5 h | cell lysates, tissue extracts, blood | 371757 | |
| JNK Activity Immunoassay Kit | Immunoblot | 40 Tests | Cell lysates | 420115 | |||
| K-LISA™ mTOR Activity Kit | 96-well plate | 96 Tests | Purified or partially purified enzyme | CBA055 | |||
| LCAT Activity Assay Kit, Fluorometric | 100 Tests | 4.5 - 8.5 h | Plasma | 428900 | |||
| Nitric Oxide Assay Kit, Colorimetric | 96-well plate | 80 Tests | 1-1.5 h | Aqueous solutions | 482650 | ||
| Nitric Oxide Assay Kit, Fluorometric | 96-well plate | 192-288 Tests | 3-5 h | Aqueous solutions | 482655 | ||
| Nitric Oxide Synthase Assay Kit | Scintillation vial | 50 Tests | picomolar range | 2 h | Tissue or cell extracts | 482700 | |
| Nitric Oxide Synthase Assay Kit, Colorimetric | 96-well plate | 96 Tests | 2 h | Plasma, serum, urine, tissue culture medium, and tissue homogenates | 482702 | ||
| OxyDNA Assay Kit, Fluorometric | 50 Tests | 4 h | Cells | 500095 | |||
| Protease Assay Kit | Cuvette or 96-well plate | 200 Tests | 10 ng/ml | 3 h | Biological samples | 539125 | |
| PTP1B Assay Kit, Colorimetric | 96-well plate | 96 Tests | 2 h | Purified enzyme | 539736 | ||
| β-Secretase Activity Assay Kit, Fluorogenic | Cuvette or 96-well plate | 100 Tests | 1.5 h | Cell lysates, tissue extracts, purified BACE | 565785 | ||
| Superoxide Anion Detection Kit, Chemiluminescent | Round-bottom polystyrene luminometer tubes | 100 Tests | 1.5 h | Cell cultures | 574590 | ||
| Total Antioxidant Status Assay Kit | Cuvette | 50 Tests | 0-25 nmol/10 µl plasma | Serum, plasma, tissue extracts, plant extracts, beverages | 615700 | ||
| Urokinase Activity Assay Kit, Fluorogenic | 96-well plate | 100 Tests | 0.01 U/ml | 0.78 - 50 U/ml | 1.5 h | Cell lysates, plasma, and serum, for screening uPA inhibitors | QIA125 |
