PS1012
PhosphoDetect™ Anti-VEGFR2 (pTyr¹2¹4) Rabbit pAb
Anti-KDR/Flk-1, Phospho-Specific (Tyr¹2¹4), Anti-VEGF Receptor 2 (pTyr¹2¹4) Rabbit pAb
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03 June 2012
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Recognizes the ~200 kDa VEGFR-2 phosphorylated at Tyr1214.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹2¹4 phosphorylation site of human VEGFR-2 | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, pH 7.3 (± 0.1). | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | NIH3T3 cells transfected with full-length, wild-type human VEGFR2 or porcine aortic endothelial (PAE) cells transfected with chimeric CSF-1/mouse VEGFR2, stimulated with CSF-1 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Irritant |
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| Data | |||
|---|---|---|---|
 Detection of mouse VEGFR2, phosphorylated at Tyr<sup>1214</sup>, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with chimeric CSF-1/mouse VEGFR2, stimulated with CSF-1 (lanes 1-4,6) or left unstimulated (lane 5). Primary antibody: PhosphoDetect™ Anti-VEGFR2 (pTyr<sup>1214</sup>) Rabbit pAb (Cat. No. PS1012) (0.5 μg/ml) pre-incubated without peptide (lanes 1,5,6), with non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence. |
