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71177  Rosetta-gami™ B Competent Cell Set

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18 四月 2014

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Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotypes:
Rosetta-gami B
F ompT hsdSB (rB mB) gal dcm lacY1 ahpC gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

Rosetta-gami B(DE3)
F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)

Rosetta-gami B(DE3)pLysS
F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR)
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產品資訊
Avoid freeze/thaw
Components
2 × 0.2 mlRosetta-gami B Competent Cells
2 × 0.2 mlRosetta-gami B(DE3) Competent Cells
2 × 0.2 mlRosetta-gami B(DE3)pLysS Competent Cells
4 × 2 mlSOC Medium
2 ngTest Plasmid
儲存和運送資訊
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Germany, 2014


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