AP1063
PhosphoDetect™ Anti-PDH-E1α (pSer232) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer232 Rabbit pAb
For general questions please contact our Customer Service:
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Phone: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-mail: customer.service@merckbio.eu
04 June 2012
loading
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser232 phosphorylation site of human PDH-E1α | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
||
| Avoid freeze/thaw | Yes | ||
| Positive control | HEK293 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Data | |||
|---|---|---|---|
 Detection of human PDH-E1α, phosphorylated at Ser<sup>293</sup>, Ser<sup>300</sup>, and Ser<sup>232</sup> by immunoblotting. Samples: Whole tissue extract from mouse liver (20 µg) left untreated (lane 1) or treated with dichloroacetate (DCA, 5 mM for 4 h) (lane 2). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) PhosphoDetect™ Anti PDH-E1α (pSer<sup>300</sup>) Rabbit pAb (Cat. No. AP1064) PhosphoDetect™ Anti PDH-E1α (pSer<sup>232</sup>) Rabbit pAb (Cat. No. AP1063) all used at 0.25 µg/ml and Anti-PDHE-1α. Detection: chemiluminescence. Data courtesy of Matthew Rardin, University of California San Diego.  Detection of PDH-E1α, phosphorylated at Ser<sup>293</sup>, Ser<sup>300</sup>, and Ser<sup>232</sup> by immunocytochemistry. Samples: COS7 cells incubated in presence dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker<sup>®</sup> Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween<sup>®</sup> 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) (panel B), PhosphoDetect™ Anti-PDH-E1α (pSer<sup>300</sup>) Rabbit pAb (Cat. No. AP1064) (panel C), PhosphoDetect™ Anti-PDH-E1α (pSer<sup>232</sup>) Rabbit pAb (Cat. No. AP1063) (panel D) (0.5 µg/ml, green fluorescence) and anti-PDH-E1α antibody (green fluorescence, top 3 panels) (panel A). Detection: fluorescence (Alexa Fluor<sup>®</sup> 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Matthew Rardin, University of California San Diego. |
