AP1062
PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer293 Rabbit pAb
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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Phone: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-mail: customer.service@merckbio.eu
04 June 2012
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Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4. | ||
| Species reactivity | human, mouse, primate, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Positive control | HEK293 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
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 Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).  Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.  Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence. |
