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Cell Signaling Assay Kits Using Luminex® Technology

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Related Resources: Analyte Quarterly | Application NotesPosters
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Tips and TricksThe Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX®

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New Cell Signaling Kit
Tips and TricksMILLIPLEX® Human Ras-Raf Oncoprotein 6 Plex Kit for Luminex® xMAP® Technology

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AACR 2020 Poster
AACR 2020 PosterSpecific detection of mutant Ras oncoproteins in cell and tissue lysates by multiplex immunoassay

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Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/MEI-Wallchart-Milliplex-Map.jpgCancer and Metastasis Signaling Pathways Wall Chart

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App Note
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Bcl-2 Family Poster

Learn how Bcl-2 family multiplex panels with their great sensitivity, specificity and reproducibility provide a powerful tool for studying the underlying mechanisms involving Bcl-2 family members.Learn more about 48-682MAG and 48-683MAG.

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Measure activation of cell signaling pathways, using MILLIPLEX® bead-based multiplex assays.
Multiplexing, using the Luminex xMAP® platform, provides faster answers to your intracellular pathway questions compared to traditional Western blots, mass spectrometry, and radioactive phosphorylation assays that require large amounts of sample.

Learn more about Luminex technology

MILLIPLEX® intracellular pathway multiplex kits and panels are analytically verified using the same stringent performance criteria used to manufacture our circulating biomarker kits, and they are optimized to yield maximum data from your precious samples. Our cell signaling assays enable accurate relative quantitation of both total and phosphorylated forms of signaling proteins, revealing connections and crosstalk within your pathways of interest.

Accelerate Your Signaling Research using MILLIPLEX® Multiplex Assays.

Rely on the quality we build into each kit to produce results you trust. In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our verification process: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Our analytically verified intracellular pathway assay kits provide:
  • Stimulated and unstimulated cell lysates provided to qualify assay performance. 
  • Premixed magnetic beads to capture analytes of interest 
  • Detection antibody cocktails designed to yield consistent analyte profiles within panel
Some examples of the intracellular pathway assay kits in our portfolio are listed below.

Cell Signaling Phosphoprotein + Total 2 Plex Assays

  • Directly compare the phosphoprotein vs. total protein levels in your sample by reading them in the same well
  • Combine with other phospho/total 2 plex assays to study phosphorylation and total expression of multiple proteins simultaneously
  • Get relatively quantitative fold-increase data from your multiplexed signaling assay kits
Use the Analyte Finder to browse all Cell Signaling Phosphoprotein + Total 2 Plex Assays.

Cell Signaling Phosphoprotein and Total Multiplex Premixed Assays

  • Measure multiple phosphoproteins or total proteins within or across pathways in a single sample
  • Get relatively quantitative fold increase data from your multiplexed signaling assay kits
Use the Analyte Finder to browse all Cell Signaling Phosphoprotein + Total Multiplex Premix Assays.

Singleplex MAPmate™ Loading Control Kits

Plex loading control MAPmate™ assays into existing MILLIPLEX® Cell Signaling Panels to enhance the panel within the guidelines provided in the protocols. Our Cell Signaling Buffer and Detection Kit (48-602MAG), may be purchased if additional buffers are required. Our custom immunoassay team can assist with differently configured cell signaling assays. For more information on this program, contact your sales specialist. Each of the loading control MAPmate™ assays include:
  • Capture Antibody (bead-conjugated)
  • Detection Antibody (biotin-conjugated)
Our Cell Signaling Buffer and Detection Kit (48-602MAG) contains:
  • MILLIPLEX® Lysis Buffer (Cat. # 43-040)
  • MILLIPLEX® Assay Buffer 2 (Cat. # 43-041)
  • MILLIPLEX® Streptavidin-Phycoerythrin (Cat. # 45-001H)
  • MILLIPLEX® Amplification buffer (Cat. # 43-024A)
  • MILLIPLEX® HeLa Cell Lysate:Unstimulated (Cat. # 47-205)
  • 96-well plate and 2 sealers

To expand the power of your intracellular research, we also offer circulating MILLIPLEX® multiplex assays for:
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/luminex-magpix2.jpgNew to multiplexing?
Click here to learn more about how Luminex technology works.

Download the Analyte Quarterly brochure for a complete, updated list of assays.

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