MABC303
Anti-PELP1 Antibody, clone 2F11.1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2F11.1, monoclonal
Application:
ICC, WB
Citations:
-
conjugate
unconjugated
Quality Segment
antibody form
purified antibody
clone
2F11.1, monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2aκ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant protein corresponding to human PELP1.
~160 kDa observed. PELP1/MNAR has a predicted molecular weight of 120 kDa and an isoelectric point of 4.30, but because of the overall negative charge and excessive amount of prolines, the protein migrates on SDS-PAGE as a 160-kDa protein (Rajhans, R., and Vadlamudi, R.K. (2006). Clin. Exp. Metastasis. 23(1):1-7).
Application
Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected PELP1 in HeLa and A431 cells.
Physical form
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PELP1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PELP1 in 10 µg of HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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