OP10F
Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate
liquid, clone 9E10, Calbiochem®
Product Name
Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate, liquid, clone 9E10, Calbiochem®
biological source
mouse
antibody product type
primary antibodies
clone
9E10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat (weakly), human, mouse (weakly)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
protect from light
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Analysis Note
Negative Control
HT1080 cells
HT1080 cells
Positive Control
HL-60 cells or lung carcinoma
HL-60 cells or lung carcinoma
Application
Flow Cytometry (20 µg/ml)
Frozen Sections (use Cat. No. OP10, OP10L, or OP10T)
Immunoblotting (use Cat. No. OP10, OP10L, or OP10T)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP10, OP10L, or OP10T)
Paraffin Sections (use Cat. No. OP10, OP10L, or OP10T)
Frozen Sections (use Cat. No. OP10, OP10L, or OP10T)
Immunoblotting (use Cat. No. OP10, OP10L, or OP10T)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP10, OP10L, or OP10T)
Paraffin Sections (use Cat. No. OP10, OP10L, or OP10T)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Conjugated to FITC. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
Recognizes the ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
This Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-Myc (Ab-1).
Immunogen
Epitope: within amino acids 410-419
Human
a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
Other Notes
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. For immunoblotting, paraffin sections (colorimetric) Cat. No. OP10, OP10L, and OP10F are available. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hiroyuki Kawagoe et al.
Cancer research, 67(22), 10677-10685 (2007-11-17)
N-MYC encodes a basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that is frequently overexpressed in human neuroblastoma. N-MYC overexpression has also been reported in human acute myeloid leukemias (AML), which we show here is a frequent event. Myeloid cells in N-Myc-overexpressing
Judit Garriga et al.
Molecular and cellular biology, 23(15), 5165-5173 (2003-07-16)
CDK9 is a CDC2-related kinase and the catalytic subunit of the positive-transcription elongation factor b and the Tat-activating kinase. It has recently been reported that CDK9 is a short-lived protein whose levels are regulated during the cell cycle by the
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