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200275 Berberine Chloride - CAS 633-65-8 - Calbiochem

A highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450 pM in a saturation binding assay, and EC₅₀ = 140 pM over EC₅₀ = 2.1 nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Berberine

200275
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
633-65-8C₂₀H₁₈NO₄+Cl-

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200275-25MG
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      Description
      OverviewA highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450 pM in a saturation binding assay, and EC50 = 140 pM over EC50 = 2.1 nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay). Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-β-galactosidase cell line (IC50 = 2 nM), but not in control cells. Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4+ T-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice.
      Catalogue Number200275
      Brand Family Calbiochem®
      SynonymsBerberine
      References
      ReferencesKinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
      Kong, W., et al. 2004, Nature Med. 10, 1344.
      Dimlich, R. et al. 1980, Stain Technol. 55, 217.
      Product Information
      CAS number633-65-8
      FormYellow powder
      Hill FormulaC₂₀H₁₈NO₄+Cl-
      Chemical formulaC₂₀H₁₈NO₄+Cl-
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC (NMR if no UV signal)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Berberine Chloride - CAS 633-65-8 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Berberine Chloride - CAS 633-65-8 - Calbiochem Certificates of Analysis

      TitleLot Number
      200275

      References

      Reference overview
      Kinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
      Kong, W., et al. 2004, Nature Med. 10, 1344.
      Dimlich, R. et al. 1980, Stain Technol. 55, 217.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-April-2012 JSW
      SynonymsBerberine
      DescriptionA highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450 pM in a saturation binding assay, and EC50 = 140 pM over EC50 = 2.1 nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay). Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-β-galactosidase cell line (IC50 = 2 nM), but not in control cells. Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4+ T-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice.
      FormYellow powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number633-65-8
      Chemical formulaC₂₀H₁₈NO₄+Cl-
      Structure formulaStructure formula
      Purity≥95% by HPLC (NMR if no UV signal)
      SolubilityDMSO (10 mg/ml) or H₂O (1 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesKinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
      Kong, W., et al. 2004, Nature Med. 10, 1344.
      Dimlich, R. et al. 1980, Stain Technol. 55, 217.