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234155

Collagenase, Type II, Clostridium histolyticum

Collagenase, Type II, Clostridium histolyticum, is a crude collagenase preparation that contains higher clostripain and tryptic activity than Type I.

Synonym(s):

Collagenase, Type II, Clostridium histolyticum

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352202
MDL number:
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Product Name

Collagenase, Type II, Clostridium histolyticum, Collagenase, Type II, Clostridium histolyticum, is a crude collagenase preparation that contains higher clostripain and tryptic activity than Type I.

form

lyophilized

specific activity

≥200 collagenase units/mg dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

solubility

aqueous buffer: soluble

foreign activity

Non-specific proteolytic ≥50 fluorescence units/mg

shipped in

ambient

storage temp.

2-8°C

Quality Level

Disclaimer

Toxicity: Harmful (C)

General description

Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.
Crude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.

Other Notes

Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys.101, 81.
One unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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