AAMT006
Mitochondrial Complex IV (Mouse) Activity Assay Kit
manufacturer/tradename
Novagen®
General description
This product has been discontinued.
Product Description
The Mitochondrial Complex IV (Mouse) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a mouse sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:
4 cytochrome c- + 4 H+ + 4 H+ (in) + O2 → 4 cytochrome c + 2 H2O + 4 H+ (out)
reduced oxidized
↑Abs 550 nm ↓Abs 550 nm
Panel Components
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.
This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitchondrial Complex IV (Mouse) Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
Product Description
The Mitochondrial Complex IV (Mouse) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a mouse sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:
Panel Components
10 ml Buffer, (72616-10ML)1 ml Detergent, (72617-1ML)1 ml Reduced Cytochrome C, (72618-1ML)1 ea Microplate, (72619-1EA)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.
This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitchondrial Complex IV (Mouse) Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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J-S Park et al.
Cell death & disease, 5, e1200-e1200 (2014-04-26)
The overproduction of reactive oxygen species (ROS) and reactive nitrogen species (RNS) has been known to contribute to the pathogenesis of noise-induced hearing loss. In this study, we discovered that in BALB/c mice pretreatment with methylene blue (MB) for 4
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