MAB1285
Anti-HLA-B27,Cln HLA-ABC-m3 (mouse monoclonal)
clone HLA-ABC-m3, Chemicon®, from mouse
Synonym(s):
Anti-AS, Anti-B-4901, Anti-HLAB
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
HLA-ABC-m3, monoclonal
Application:
FACS, ICC
Citations:
5
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
HLA-ABC-m3, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HLA-B(3106)
General description
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
Application
This Anti-HLA-B27 Antibody, clone HLA-ABC-m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
Biochem/physiol Actions
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
Physical form
Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
Preparation Note
Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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