MAB8121
Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2, and 2D4.2
Chemicon®, from mouse
Synonyme(s) :
CMV
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
1G5.2, monoclonal, 2D4.2, monoclonal, 8B1.2, monoclonal
Species reactivity:
human
Application:
IF, IHC
Citations:
3
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1G5.2, monoclonal, 2D4.2, monoclonal, 8B1.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable
isotype
IgG2a
shipped in
wet ice
Immunogen
Epitope: blend
MRC-5 infected cells with ATCC VR538 and AD169, ultrasonicated, screened by plate reduction neutralization tests.
Application
Immunohistochemistry
Immunofluorescence
Optimal working dilutions must be determined by the end user.
Immunofluorescence
Optimal working dilutions must be determined by the end user.
This Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2 & 2D4.2 is validated for use in IF, IH for the detection of Cytomegalovirus.
Biochem/physiol Actions
Reacts with Immediate early, early, and late antigen preparations. Can detect CMV infection within 2 hours post-infection exhibiting a nuclear staining which reaches peak intensity between 48 and 96 hours. This antigen is persisting and can be detected during the complete CMV infection cycle. Staining of CMV structural antigens becomes apparent at about 48 hours. Extra-nuclear staining is first seen in the region of the golgi apparatus followed subsequently by staining of viral proteins and particles in the cytoplasm.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Physical form
0.02 M PB, 0.25M NaCl, PH 7.6 with 0.1% Sodium Azide.
Format: Purified
Preparation Note
Maintain at +2-8C in convenient aliquots for up to 6 months. Do not freeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe de stockage
12 - Non Combustible Liquids
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