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Merck

MABF851

Anti-ERAP1 Antibody, clone 16A7.1

clone 16A7.1, from mouse

Synonym(s):

Endoplasmic reticulum aminopeptidase 1, A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase,

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
16A7.1, monoclonal
Application:
WB
Species reactivity:
human
Citations:
1
Technique(s):
western blot: suitable
Uniprot accession no.:
Q9NZ08

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

16A7.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001035548

UniProt accession no.

Q9NZ08

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ERAP1(51752)

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ERAP1 in 10 µg of A431 cell lysate.

Application

Detect ERAP1 using this Anti-ERAP1 Antibody, clone 16A7.1 validated for use in Western Blotting.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected ERAP1 in 10 µg of K562 cell lysate.

Biochem/physiol Actions

Expected to react with both spliced isoforms of human ERAP1 (UniProt Q9NZ08-1 & Q9NZ08-2)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Endoplasmic reticulum aminopeptidase 1 (UniProt Q9NZ08; also known as A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator) is encoded by the ERAP1 (also known as ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILSAP) gene (ORF UNQ584/PRO1154; Gene ID 51752) in human. Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a single transmembrane protein belonging to the M1 family of zinc metallopeptidases. ERAP1 consists of an N-terminal transmembrane segment (a.a. 2-21) and a large lumenal domain (a.a. 22-941), with only the N-terminus methionine exposed cytoplasmically. ERAP1 processes peptides for major histocompatibility complex (MHC) class I presentation and promotes ectodomain shedding of several cytokine receptors, including TNF-R1, IL-6R alpha chain, and IL-1 type II decoy receptor (IL-1RII). Ankylosing spondylitis (AS) is a common, highly heritable form of inflammatory arthritis. Several ERAP1 single nucleotide polymorphic (SNP) variants are reported to influence the risk of AS in individuals carrying class I MHC allele HLA-B*27 and HLA-B*40:01.
~110 kDa observed. 107.2 kDa (isoform 1) and 107.8 kDa (Isoform 2) calculated.

Immunogen

Epitope: Internal.
GST-tagged recombinant human ERAP1 internal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Peter M Bruno et al.
Nature biotechnology, 41(7), 980-992 (2023-01-03)
Identification of CD8+ T cell epitopes is critical for the development of immunotherapeutics. Existing methods for major histocompatibility complex class I (MHC class I) ligand discovery are time intensive, specialized and unable to interrogate specific proteins on a large scale.

Global Trade Item Number

SKUGTIN
MABF85104055977312898

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