C0858
Cyclooxygenase 2 human
≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein
Synonym(s):
COX-2, Prostaglandin H synthase 2
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About This Item
Product Name
Cyclooxygenase 2 human, ≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein
recombinant
expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥8000 units/mg protein
mol wt
dimer subunit mol wt 70-74 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... PTGS2(5743)
Biochem/physiol Actions
COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.
General description
KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.
Other Notes
One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.
Tagged with a six-residue histidine sequence near the N-terminus.
Physical form
Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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María J Pérez et al.
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Evidence-based complementary and alternative medicine : eCAM, 2022, 7921408-7921408 (2022-04-12)
Background. The current study aims to give a scientific origin for employing Habenaria plantaginea Lindl. as a potential candidate against nociception, inflammation, and pyrexia. The pharmacological studies were performed on crude extract and subfractions. In the gas chromatography-mass spectroscopy analysis
Masanori Sugimoto et al.
Drug development research, 77(1), 20-28 (2016-01-15)
Preclinical Research This article describes the properties of a novel topical NSAID (Nonsteroidal anti-inflammatory drug) patch, SFPP (S(+)-flurbiprofen plaster), containing the potent cyclooxygenase (COX) inhibitor, S(+)-flurbiprofen (SFP). The present studies were conducted to confirm human COX inhibition and absorption of
Muhammad Saeed Jan et al.
European journal of medicinal chemistry, 186, 111863-111863 (2019-11-20)
In recent years, drug discovery paradigm has been shifted from conventional single target inhibition toward multitarget design concept. In current research, we have reported synthesis, in-vitro, in-vivo and acute toxicity determination of N-substituted pyrrolidine-2,5-dione derivatives as multitarget anti-inflammatory agents. We
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