S7651

Sphingomyelinase from Bacillus cereus

Name: Sphingomyelinase from <I>Bacillus cereus</I>
Brand: SIGMA

lyophilized powder, ≥100 units/mg protein

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:

9031-54-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-869-9

MDL number:

MFCD00132362

EC Number:

3.1.4.12 (BRENDA, IUBMB)

Product Name

Sphingomyelinase from Bacillus cereus, lyophilized powder, ≥100 units/mg protein

form

lyophilized powder

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Quality Level

200

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

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General description

Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg²⁺-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Application

Sphingomyelinase from Bacillus cereus has been used:

in the measurement of membrane capacitance in brain slices
for testing and confirming its silkworm-killing activity
in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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[Structure and function of sphingomyelinase].

Masataka Oda

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 129(10), 1233-1238 (2009-10-03)

Bacillus cereus is one that causes of opportunistic human infections. Sphingomyelinase produced by B. cereus is assumed a virulence factor for the infection. Sphingomyelinase from Bacillus cereus (Bc-SMase) is Mg(2+)-containing metalloenzyme. Bc-SMase is a family of neutral SMase (nSMase) and

Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity

Usui K, et al.

Microbial Pathogenesis, 46(2), 59-62 (2009)

Lipid bilayers containing sphingomyelins and ceramides of varying N-acyl lengths: a glimpse into sphingolipid complexity

Jimenez-Rojo N, et al.

Biochimica et Biophysica Acta - Biomembranes, 1838(1), 456-464 (2014)

Promotion of endocytosis efficiency through an ATP-independent mechanism at rat calyx of Held terminals

Yue HY, et al.

The Journal of Physiology, 595(15), 5265-5284 (2017)

Hemolysis induced by Bacillus cereus sphingomyelinase.

Masataka Oda et al.

Biochimica et biophysica acta, 1798(6), 1073-1080 (2010-03-11)

Bacillus cereus sphingomyelinase (Bc-SMase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. We investigated the mechanism of this hemolysis in comparison to that induced by Clostridium perfringens alpha-toxin. Pertussis toxin, a Gi-specific inhibitor, N-oleoylethernolamine, a ceramidase inhibitor

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