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Merck

05-363

Anti-Cyclin E Antibody, clone HE12

clone HE12, Upstate®, from mouse

Synonym(s):

Anti-CCNE, Anti-pCCNE1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Cyclin E Antibody, clone HE12, clone HE12, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HE12, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_001238
NM_057182

UniProt accession no.

P24864

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CCNE1(898)

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated in immunoblot on human A431 lysate

Application

Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.

Biochem/physiol Actions

human p52cyclin E

General description

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
52 kDa

Immunogen

Recombinant human cyclin E

Other Notes

Replaces: 04-223

Physical form

Format: Purified

Preparation Note

2 years at -20°C or 6 months at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
Maimets, T; Neganova, I; Armstrong, L; Lako, M
Oncogene null
S M Keenan et al.
The Journal of biological chemistry, 276(25), 22404-22409 (2001-04-17)
Activation of cyclin-dependent kinase 2 (CDK2)-cyclin E in the late G(1) phase of the cell cycle is important for transit into S phase. In Chinese hamster embryonic fibroblasts (IIC9) phosphatidylinositol 3-kinase and ERK regulate alpha-thrombin-induced G(1) transit by their effects
Hae-June Lee et al.
Radiation research, 170(5), 579-590 (2008-10-31)
The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA
Nathaniel W Holton et al.
DNA repair, 66-67, 42-49 (2018-05-04)
Environmental exposures, reactive by-products of cellular metabolism, and spontaneous deamination events result in a spectrum of DNA adducts that if un-repaired threaten genomic integrity by inducing mutations, increasing instability, and contributing to the initiation and progression of cancer. Assessment of
Delphine Larrieu et al.
Cell cycle (Georgetown, Tex.), 9(19), 3984-3990 (2010-10-05)
ING2 (Inhibitor of Growth 2) is a candidate tumor suppressive protein frequently lost in human tumors. Recently, we have reported that ING2 downregulation impairs DNA replication forks progression and leads to genome instability. To better understand the tumor suppressive functions
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Global Trade Item Number

SKUGTIN
05-36304053252329081
05-363-25UG04054839350184

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