Anti-Opioid Receptor Antibody, µ, pain

45 kDa

Opiates derived from the opium poppy, Papaver somniferum, have been used for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, and µ (also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the µ opioid receptor, agonists of spinal δ opioid receptors have antinociceptive activity that is independent of µ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002).

Research Sub Category
Neuroinflammation & Pain

Detect Opioid Receptor using this Anti-Opioid Receptor Antibody, µ, pain validated for use in IC, IH & WB.

Immunohistochemistry:
1:2,500-1:5,000 dilution of a previous lot was used.

Western blot:
1:1,000 dilution of a previous lot was used.

Immunocytochemistry:
1:2,500-1:5,000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Control
Spinal cord, liver lysate

Evaluated by Western Blot on Human Liver lysate.

Western Blot Analysis:
1:500 dilution of this antibody detected Opioid Receptor on 10 µg of Human Liver lysate.

Mu Opioid receptor.

Other species have not been tested.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Epitope: C-terminus

Synthetic peptide from the C-terminus of rat Mu Opioid receptor.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rabbit polyclonal serum in buffer containing 0.05% sodium azide.

Unpurified

Stable for 1 year at -20ºC from date of receipt.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany