Product Name
Anti-Digoxigenin, from mouse IgG1κ (clone 1.71.256)
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 1.71.256, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
technique(s)
ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL
isotype
IgG1κ
storage temp.
2-8°C
Quality Level
Analysis Note
There are no cross reactivities known.
Application
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Immunofluorescence staining
- FISH (fluorescent in situ hybridization)
Biochem/physiol Actions
In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
The monoclonal antibody reacts with free and bound digoxigenin.
Features and Benefits
- Specific to free and bound digoxigenin
- The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
General description
Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications
phosphate buffered saline, pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications
phosphate buffered saline, pH 7.4
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Carla Winterling et al.
RNA biology, 11(1), 66-75 (2014-01-21)
A growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. Besides small RNAs, the diverse class of long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance for infection, a
Ilyas Chachoua et al.
Nature communications, 13(1), 204-204 (2022-01-13)
Abnormal WNT signaling increases MYC expression in colon cancer cells in part via oncogenic super-enhancer-(OSE)-mediated gating of the active MYC to the nuclear pore in a poorly understood process. We show here that the principal tenet of the WNT-regulated MYC
Jiaqiong Wang et al.
Journal of neuropathology and experimental neurology, 72(8), 768-781 (2013-07-19)
Much of the morbidity after traumatic brain injury (TBI) is associated with traumatic axonal injury (TAI). Although most TAI studies focus on corpus callosum white matter, the visual system has received increased interest. To assess visual system TAI, we developed
Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction
<BIG>Lion T and Haas OA</BIG>
Analytical Chemistry, 188, 335-337 (1990)
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