C7729

Anti-Caspase 9 antibody produced in rabbit

Name: Anti-Caspase 9 antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-APAF-3, Anti-APAF3, Anti-ICE-LAP6, Anti-MCH6, Anti-PPP1R56

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All Photos(2)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01864176

Product Name

Anti-Caspase 9 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35-47 kDa (two bands)

species reactivity

human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart
immunoprecipitation (IP): 10 μg using HeLa mitochondrial RIPA lysate (500 μg)
microarray: suitable
western blot: 1:300 using whole extract of human Jurkat cells

UniProt accession no.

P55211

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CASP9(842)
rat ... Casp9(58918)

Application

Anti-Caspase 9 antibody produced in rabbit has been used in western blot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Caspase 9/CASP9 is essential for apoptosis during normal development of the murine central nervous system and plays a role in controlling tumor development. It functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 can also be activated by granzyme B.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Caspase 9 is developed in rabbit using a synthetic peptide corresponding to amino acid of human procaspase 9 with N-terminal added lysine conjugated to keyhole limpet hemocyanin (KLH) with glutaraldehyde. Caspase 9/CASP9 (Mch6, ICE-LAP6, APAF3) is a member of the caspase 2 subfamily and is classified together with caspases 8 and 10 as an initiator caspase. It is a cysteine containing aspartate specific protease, made up of 416 amino acids, of which 13 are cysteine. CASP9 is located on human chromosome 1p36.21.

Immunogen

synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Acidic extracellular pH shifts colorectal cancer cell death from apoptosis to necrosis upon exposure to propionate and acetate, major end-products of the human probiotic propionibacteria

Lan A, et al.

Apoptosis, 12(3), 573-591 (2007)

Memantine - neuroprotective drug in aging brain.

Dominika Karolczak et al.

Polish journal of pathology : official journal of the Polish Society of Pathologists, 64(3), 196-203 (2013-10-30)

Aging is the process of progressive accumulation of changes over time, which is additionally connected with increasing susceptibility to some diseases and ultimately leads to death. Aging is associated mainly with loss of permanent cells, e.g. in heart, skeletal muscle

8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells.

Qing Ding et al.

Molecular medicine reports, 12(4), 5100-5108 (2015-07-08)

8‑bromo‑7‑methoxychrysin (BrMC), a novel chrysin analog, was reported to have anti‑cancer activities. The aim of the present study was to investigate the molecular mechanism of 8‑bromo‑7‑methoxychrysin (BrMC)‑induced apoptosis via the Akt/forkhead box O3a (FOXO3a) pathway in cisplatin (DDP)‑sensitive and ‑resistant

Genetic screening and functional analysis of CASP 9 mutations in a Chinese cohort with neural tube defects

Liu XZ, et al.

CNS Neuroscience & Therapeutics, 24(5), 394-403 (2018)

Allicin inhibits oxidative stress-induced mitochondrial dysfunction and apoptosis by promoting PI3K/AKT and CREB/ERK signaling in osteoblast cells.

Guoliang Ding et al.

Experimental and therapeutic medicine, 11(6), 2553-2560 (2016-06-11)

Osteoporosis is a disease of the skeleton that is characterized by the loss of bone mass and degeneration of bone microstructure, resulting in an increased risk of fracture. Oxidative stress, which is known to promote oxidative damage to mitochondrial function

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