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Luminex® Multiplexing Instruments

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Brian McFarlin Click on image to view video
Achieve low concentration human serum cytokines using MILLIPLEX® High-Sensitivity Assays. In a new, step-by-step article in JOVE (Journal of Visualized Experimentation), Dr. McFarlin demonstrates how you can measure multiple, low-concentration cytokines in a few easy steps:
  • Serum collection
  • Total protein/lipid analysis
  • Multiplexed detection with MILLIPLEX® High-Sensitivity Cytokine Assay Panel
  • Data analysis with MILLIPLEX® Analyst 5.1 software
Luminex® xMAP® technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver both high density and high throughput multiplexed assay capabilities at the same time. As a Luminex® partner, we offer the entire portfolio of multiplexing instruments, including Luminex 200™, FLEXMAP 3D® and xMAP® INTELLIFLEX platforms, covering the full range of multiplexing capabilities. Our MILLIPLEX® multiplex assays are compatible with all of the Luminex® xMAP® platforms.

Luminex® Instruments Comparison
Merck:/Freestyle/BI-Bioscience/Protein-Detection/luminex-images/lx200.png Merck:/Freestyle/BI-Bioscience/Protein-Detection/luminex-images/fm3d.png Merck:/Freestyle/BI-Bioscience/Protein-Detection/luminex-images/iflex.png
Instrument Luminex 200™ FLEXMAP 3D® xMAP® INTELLIFLEX RUO
Cat No 40-012 40-014 INTELLIFLEX-RU0
Software xPONENT® 4.3 xPONENT® 4.3 xMAP® INTELLIFLEX Software
Optic Lasers/APDs/PMTs Lasers/APDs/PMTs Lasers/APDs/PMTs
Hardware Flow Cytometry based Flow Cytometry based Flow Cytometry based
Bead Compatibility Magnetic and non-Magnetic Magnetic and non-Magnetic Magnetic and non-Magnetic
Multiplex Capacity 100 (80 for MagPlex) 500 500
Read Time 96 well plate ~40 mins 96 well plate ~20 mins
384-well plate ~75 min
96 well plate ~20 mins
384-well plate ~75 min
Applications Protein/Nucleic Acid Protein/Nucleic Acid Protein/Nucleic Acid
Dynamic Range 3.5 logs 4.5 logs 5.5 logs
Microtiter Plate 96 well 96 well & 384 well 96 well & 384 well
Footprint including PC 115 cm x 60 cm x 50 cm 110 cm x 62 cm x 63 cm 58.4 cm W x 61 cm D x 76.2 cm H
Weight (Analyzer) 49 kg (113 lbs.) 77.1 kg (170 lbs.) 54.4 kg (120 lbs)
Automation Compatible Yes Yes Yes
Analysis Software Belysa™ Immunoassay Curve Fitting Software
CFR 21 Part 11 Yes Yes Yes
Automation Yes Yes Yes
Security Yes Yes Yes
LIS Yes Yes Yes