Solid Phase Extraction with LiChrolut® EN (200 mg)

Solvents	A: Methanol (LiChrosolv® Cat. No. 1.06007), B: Acetonitrile (LiChrosolv® Cat. No. 1.00030); C: Water (LiChrosolv® Cat. No. 1.15333).
Sample pretreatment	Filtrate if necessary and add approx. 5 g NaCl /1 L water sample. Conditioning of extraction column: 3 mL A, 3 mL B, 10 mL C, do not allow column to dry out!
Sample application	Pass 1 L water sample through the extraction column within 1 hour by using LiChrolut® extraction unit (Cat. No. 1.19851) which is connected to the water sample reservoir with PTFE hose (Cat. No. 1.22143) and a steel capillary.
Drying step	10 min by means of nitrogen and LiChrolut® drying unit (Cat. No. 1.19852).
Elution step	1 x 2 mL, then 1 x 3 mL A/B (1:1), collect in a conical flask and gently evaporate the solvent by means of nitrogen up to an end volume of 0.5 mL. Fill up to 1.0 mL with C. Subsequently, filtrate sample into a 1.5 mL sample vial through a 0.2 μm membrane filter.