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MAB19292 Anti-ADAM2 Antibody, clone 9D2.2

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MAB19292
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB19292
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ADAM2 Antibody, clone 9D2.2
      Alternate Names
      • Fertilin beta
      References
      Product Information
      FormatPurified
      PresentationLiquid at 1 mg/mL in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ADAM2 Antibody, clone 9D2.2 is validated for use in ELISA, WB for the detection of ADAM2.
      Key Applications
      • ELISA
      • Western Blotting
      Applications Not Recommended
      • Immunohistochemistry
      Application NotesWestern blot: 1:500 - 1:1,000 dilution for detected against sperm lysates, using standard alkaline phosphatase with NBT/BCIP detection. Chemiluminescent detection may permit higher dilutions.

      Not effective for immunohistochemistry.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant mouse fertilin produced by baculovirus expressin in SF9 cells.
      Clone9D2.2
      HostMouse
      SpecificityRecognizes mouse fertilin beta by immunoblot, staining of bands in a 46-48 kDa range (multiple bands are due to variable protein glycosylation).
      IsotypeIgG2b
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions.
      Gene Symbol
      • ADAM2
      • PH30
      • CRYN1
      • FTNB
      • PH30-beta
      • CRYN2
      • PH-30b
      • PH-30
      • CT15
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99965 # Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
      SIZE: 735 amino acids; 82457 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
      DOMAIN:SwissProt: Q99965 A tripeptide motif (FEE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
      PTM: The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.
      SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain.
      MISCELLANEOUS: In mammals, exists as a heterodimer composed of an alpha and beta subunits. In human, fertilin subunit alpha is a pseudogene.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB19292 04053252265778

      Documentation

      Anti-ADAM2 Antibody, clone 9D2.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ADAM2 Antibody, clone 9D2.2 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-FERTILIN BETA [ADAM 2] - 3385695 3385695
      MOUSE ANTI-FERTILIN BETA [ADAM 2] - 4185151 4185151
      MOUSE ANTI-FERTILIN BETA [ADAM 2] -2679415 2679415
      MOUSE ANTI-FERTILIN BETA [ADAM 2] -2780431 2780431
      MOUSE ANTI-FERTILIN BETA [ADAM 2] MONOCLONAL ANTIBODY 3154982
      MOUSE ANTI-FERTILIN BETA [ADAM 2] MONOCLONAL ANTIBODY - 2011454 2011454
      MOUSE ANTI-FERTILIN BETA [ADAM 2] MONOCLONAL ANTIBODY - 2280860 2280860
      MOUSE ANTI-FERTILIN BETA [ADAM 2] MONOCLONAL ANTIBODY - 2986160 2986160
      Mouse Anti-Fertilin Beta (Adam 2) - 3927174 3927174

      References

      Reference overviewPub Med ID
      Prss37 is required for male fertility in the mouse.
      Shen, C; Kuang, Y; Liu, J; Feng, J; Chen, X; Wu, W; Chi, J; Tang, L; Wang, Y; Fei, J; Wang, Z
      Biology of reproduction  88  123  2013

      Show Abstract
      23553430 23553430
      Identification of heat shock protein 5, calnexin and integral membrane protein 2B as Adam7-interacting membrane proteins in mouse sperm.
      Cecil Han,Inju Park,Boyeon Lee,Sora Jin,Heejin Choi,Jun Tae Kwon,Yong-il Kwon,Do Han Kim,Zee Yong Park,Chunghee Cho
      Journal of cellular physiology  226  2011

      Show Abstract
      20945367 20945367
      Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta
      Nishimura, H, et al
      Dev Biol, 233:204-13 (2001)  2001

      11319869 11319869

      Data Sheet

      Title
      MOUSE ANTI-FERTILIN BETA [ADAM 2]