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MAB3786-25UG Anti-APC CT, clone C-APC 28.9

Anti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.

Anti-APC CT, clone C-APC 28.9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: APC, DP2.5, FAP, GS, DP3, DP2, FPC UniProt Number: P25054
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MAB3786-25UG
25 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIF, IP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3786-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-APC CT, clone C-APC 28.9
      Alternate Names
      • Adenomatous Polyposis Coli Protein
      Background InformationThe adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. C-APC 28.8 can be used for APC expression and detection of APC mutations.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: colon cell line HCT116
      PresentationPurified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot

      Immunohistochemistry (Frozen sections)

      Immunofluorescence

      Immunoprecipitation

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman APC C-terminal fusion with MBP (maltose binding protein).
      EpitopeC-terminus
      CloneC-APC 28.9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes human APC, molecular weight is approximately 310kDa.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
      Gene Symbol
      • APC
      • DP2.5
      • FAP
      • GS
      • DP3
      • DP2
      • FPC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25054 # Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. APC activity is correlated with its phosphorylation state.
      SIZE: 2843 amino acids; 311646 Da
      SUBUNIT: Forms homooligomers. Interacts with DIAPH1 and DIAPH2 (By similarity). Interacts with PDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with the N- terminus of ARHGEF4, and the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Found in a complex consisting of ARHGEF4, APC and CTNNB1.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      PTM: Phosphorylated by GSK3B.
      DISEASE: SwissProt: P25054 # Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years. & APC mutations have led to some interesting observations. (1) the great majority of the mutations found to date would result in truncation of the APC product. (2) almost all the mutations have occurred within the first half of the coding sequence, and somatic mutations in colorectal tumors are further clustered in a particular region, called MCR (mutation cluster region). (3) most identified point mutations in the APC gene are transitions from cytosine to other nucleotides. (4) the location of germline mutations tends to correlate with the number of colorectal polyps in FAP patients. Inactivation of both alleles of the APC gene seems to be required as an early event to develop most adenomas and carcinomas in the colon and rectum as well as some of those in the stomach. & Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also called familial infiltrative fibromatosis (FIF). It is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis. & Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome). & Defects in APC are a cause of Turcot syndrome [MIM:276300]. Turcot syndrome is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
      SIMILARITY: SwissProt: P25054 ## Contains 7 ARM repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3786-25UG 04054839350399