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ABC974-I-25UG Anti-ATG8

Anti-ATG8 Antibody, Cat. No. ABC974-I, is a rabbit polyclonal antibody that detects ATG8a and ATG8b and has been tested for use in Western Blotting.

ABC974-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC974-I-25UG
      DescriptionAnti-ATG8
      Alternate Names
      • Autophagy-related 8a
      • isoform A and Autophagy-related 8b
      • isoform B
      Background InformationAutophagy-related 8a, isoform A and Autophagy-related 8b, isoform B (UniProt: Q9W2S2 and Q9VEG5, respectively) are encoded by the ATG8a and ATG8b genes (Gene ID: 32001 and 42132, respectively) in Drosophila melanogaster. Atg8 is a highly conserved and ubiquitously expressed protein that is upregulated under various stress conditions. Atg8 genes of multicellular animals is divided into three subfamilies LC3, GABARAP, GATE16 (depending on the animal). LC3 is the mammalian ortholog of Drosophila Atg8. Atg8 It shares a strong structural similarity to ubiquitin, despite the lack of similarity in amino acid sequence. The ubiquitin core of the Atg8 consists of a four-stranded central beta sheet, where the two central strands are parallel to each other and the two outer strands are antiparallel to the central strands. Between these strands are two alpha helices with alpha3 between beta2 and beta3 and alpha4 between beta3 and beta4. In resting cells, the majority of Atg8 is in found in an unlipidated state (Atg8-I) and during autophagy it is conjugated with a lipid moiety (Atg8-II). The lipidated form is the one that is recruited to autophagosomal membranes. The level of Atg8-II is an indicator of autophagosome activation. (Ref.: Shpilka, T., et al. (2011). Genome Biology. 12(7); 226).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ATG8 Antibody, Cat. No. ABC974-I, is a rabbit polyclonal antibody that detects ATG8a and ATG8b and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptides corresponding to 15 amino acids from the N-terminal region of Drosophila melanogaster ATG8a and ATG8b.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects ATG8a and ATG8b in Drosophila melanogaster. It targets an epitope within 15 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Drosophila
      Species Reactivity NoteDrosophila.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATG8a
      • ATG8b
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~ 14 kDa observed; 14.39 and 14.46 kDa calculated for ATG8a and ATG8b, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DSL2 cell lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ATG8a and ATG8b in DSL2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC974-I-25UG 04061841099024