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ABF1055-25UL Anti-Adenovirus 5 packaging protein 52K

Anti-Adenovirus 5 packaging protein 52K, Cat. No. ABF1055, is a rabbit polyclonal antibody that detects Adenovirus packaging protein 52K.and is is tested for use in Western Blotting and Immunofluorescence.

ABF1055-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABF1055-25UL
      DescriptionAnti-Adenovirus 5 packaging protein 52K
      Alternate Names
      • Packaging protein 3
      • L1-52/55 kDa protein
      Background InformationPackaging protein 3 (UniProt: P04496; also known as L1-52/55 kDa protein, Packaging protein 52K) is encoded by the L1 gene (Gene ID: 2652993) in human Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. The 52 kDa packaging protein of human adenovirus 5 is part of the genome packaging machinery that consists of polypeptides IIIa, IVa2, L4 33k, and L4 22k. It is expressed in the early phase and late phase of viral replicative cycle. Its role during assembly consists in tethering the viral core to the icosahedral shell wherein maturation proceeds simultaneously with packaging, before the viral particle is sealed. Its adenovirus protease consensus cleavage site is localized at amino acids 351-352. It plays a critical role in the coordinated assembly of infectious progeny particles and contributes to the specificity of packaging, possibly by interacting with capsid protein IIIa. It is proteolytically cleaved at different sites during virion maturation. It is reported to be present in empty particles in its full-length form but is absent from the mature virion. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antisera without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Adenovirus 5 packaging protein 52K, Cat. No. ABF1055, is a rabbit polyclonal antibody that detects Adenovirus packaging protein 52K.and is is tested for use in Western Blotting and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Adenovirus packaging protein 52K in immunofluorescence application. ( Charman, M., et al. (2023). Nature. 616(7956):332-3380).

      Western Blotting Analysis: A 1:2,500 dilution from a representative lot detected Adenovirus packaging protein 52K in lysates from HeLa cells infected with Human adenovirus type 5. (Courtesy of Dr. Patrick Hearing, Stony Brook University).

      Western Blotting Analysis: A representative lot detected Adenovirus packaging protein 52K in Western Blotting application. ( Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged full-length, recombinant packaging protein 52K from human Adenovirus 5.
      EpitopeUnknown
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Packaging protein 52K of Human Adenovirus 5.
      IsotypeIgG
      Species Reactivity
      • Virus
      Species Reactivity NoteAdenovirus 5
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • L1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~52 kDa observed; 47.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from Human adenovirus type 5 infected HeLa cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected packaging protein 52K from human adenovirus 5 in lysate from HeLa cells infected with Human adenovirus type 5, but not in lysate from uninfected HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF1055-25UL 04065271174931