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ABC22 Anti-Atg4D Antibody

Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).

Anti-Atg4D Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ATG4D, APG4D, AUTL4 UniProt Number: Q86TL0
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ABC22
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rhesus MacaqueWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC22
      DescriptionAnti-Atg4D Antibody
      Alternate Names
      • Cysteine protease ATG4D
      • AUT-like 4 cysteine endopeptidase
      • Autophagin-4
      • Autophagy-related cysteine endopeptidase 4
      • Autophagy-related protein 4 homolog D
      Background InformationAtg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue.

      Western Blot Analysis: 1 µg/mL from a representative lot detected Atg4D on 10 µg of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Atg4D at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat.
      Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAutophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq].
      Gene Symbol
      • ATG4D
      • APG4D
      • AUTL4
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

      ENZYME REGULATION: Inhibited by N-ethylmaleimide.

      SUBCELLULAR LOCATION: Cytoplasm Probable.

      TISSUE SPECIFICITY: Mainly expressed in skeletal muscle and, to a lower extent, in testis.

      SEQUENCE SIMILARITIES: Belongs to the peptidase C54 family.
      Molecular Weight~56 kDa observed.
      The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected Atg4D in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC22 04053252632136