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ABC947-25UG Anti-Bif-1

Detect using this rabbit polyclonal Anti-Bif-1 Antibody, Cat. No. ABC947-25UG, validated for use in Western Blotting and Western Blotting and Immunohistochemistry (Paraffin).

Research Category: Apoptosis & Cancer Gene Symbol: SH3GLB1, KIAA0491, CGI-61 UniProt Number: Q9Y371
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ABC947-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC947-25UG
      DescriptionAnti-Bif-1
      Alternate Names
      • Endophilin-B1
      • Bax-interacting factor 1
      • SH3 domain-containing GRB2-like protein B1
      Background InformationEndophilin-B1 (UniProt: Q9Y371; also known as Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1) is encoded by the SH3GLB1 (KIAA0491; CGI-61) gene (Gene ID: 51100) in human.
      Bif-1 is a member of the endophilin protein family that forms a complex with Beclin-1 through ultraviolet irradiation resistant-associated gene (UVRAG) and promotes the activation of the class III PI3 kinase, Vps34, in mammalian cells. It is highly expressed in heart, skeletal muscle, kidney, and placenta and is detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung, and peripheral blood leukocytes. Loss of Bif-1 significantly suppresses PI3KC3 activation and the formation of autophagosome in both HeLa cells and mouse embryonic fibroblasts (MEFs). Bif-1 is reported to be involved in mitochondrial fission and coat protein complex I (COPI)-vesicle formation. Bif-1 contains a BAR domain at the N-terminal (aa 27-261) and a SH3 domain at the C-terminal (305-365), which is required for interaction with UVRAG. Bif-1 may be present as a homodimer or as a heterodimer with Endophilin-B2 (SH3GLB2). Bif-1 can be phosphorylated at Threonine 145 by Cdk5 and this phosphorylation is required for autophagy induction in starved neurons. Three isoforms of Bif-1 have been reported that are generated by alternative splicing. Isoform 1 acts as pro-apoptotic molecule in fibroblasts and is involved in caspase-independent apoptosis during nutrition starvation and in the regulation of autophagy. Isoform 2 has an anti-apoptotic role in neuronal cells and is involved in maintenance of mitochondrial morphology and promotes neuronal viability. (Ref.: Takahashi, Y et al (2009). Cell Death Differen. 16(7), 947-955).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect using this rabbit polyclonal Anti-Bif-1 Antibody, Cat. No. ABC947-25UG, validated for use in Western Blotting and Western Blotting and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 2 µg/mL  from a representative lot detected Bif-1 in 10 µg of Jurkat cells and human kidney lysates.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Bif-1 in human placenta, human skin, human small intestine, and human testis tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Bax interacting factor 1 (Bif-1).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Bax interacting facor 1 (Bif-1) in human cells. it targets an epitope within 15 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • SH3GLB1
      • KIAA0491
      • CGI-61
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
      Western Blotting Analysis: 2 µg/mL of this antibody detected Bif-1 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC947-25UG 04054839441608

      Documentation