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MABF2005-100UG Anti-CCR5 (CD195) Antibody, clone HEK/1/85a/7a

Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat. No. MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry.

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MABF2005-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2005-100UG
      DescriptionAnti-CCR5 (CD195) Antibody, clone HEK/1/85a/7a
      Alternate Names
      • C-C chemokine receptor type 5
      • C-C CKR-5
      • CC-CKR-5
      • CCR-5
      • CCR5
      • CHEMR13
      • HIV-1 fusion coreceptor
      • CD195
      Background InformationC-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human. CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines. It is also shown to be highly expressed in spleen, thymus, and on THP-1 and KG-1a cells. Lower expression is observed in ovary and lung. CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels. It also plays a role in the control of granulocytic lineage proliferation or differentiation. CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2. It also couples to Gi class of G-proteins to inhibit cAMP production and stimulate calcium release. CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells. It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES. Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus. (Ref.: Blanpain, C., et al. (2002). Receptors Channels. 8(1);19-31).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CCR5 (CD195), clone HEK/1/85a/7a, Cat. No. MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).

      Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
      Biological Information
      ImmunogenCHO cells transfected with full length human CCR-5 (CD195).
      Epitopeextracellular domain
      CloneHEK/1/85a/7a
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCR5
      • CMKBR5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight40.52 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in wrote RBL-2H3 cells stably transfection with CCR5.

      Flow Cytometry Analysis (FC): 1 µg of this antibody detected C-C chemokine receptor type 5 (CD195) in one million CCR-5 transfected RBL-2H3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2005-100UG 04054839607868