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MABC1764-25UG Anti-CEMIP Antibody, clone PW-3

Anti-CEMIP, clone PW-3, Cat. No. MABC1764, is a mouse monoclonal antibody that detects CEMIP and is tested for use in Immunohistochemistry and Western Blotting.

MABC1764-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1764-25UG
      DescriptionAnti-CEMIP Antibody, clone PW-3
      Alternate Names
      • Cell migration-inducing and hyaluronan-binding protein
      • CCSP1
      Background InformationCell migration-inducing and hyaluronan-binding protein (UniProt: Q8WUJ3; also known as EC:3.2.1.35, CCSP1, CEMIP) is encoded by the CEMIP (also known as KIAA1199) gene (Gene ID: 57214) in human. CEMIP is highly glycosylated protein that is widely distributed in the cytoplasm and is localized in endoplasmic reticulum, endosomes, cell membrane, and in exosomes. It is also abundantly secreted. It is synthesized with a signal peptide (aa 1-30) that is essential for its proper translocation, hyaluronic acid (HA) degradation activity, and secretion. It mediates depolymerization of HA via the cell membrane-associated clathrin-coated pit endocytic pathway. It hydrolyzes high molecular weight HA to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. CEMIP contains two GG domains, one G8 domain (aa 44-166), and four PbH1 domains (aa 572-819). The G8 domain contains eight conserved glycine residues and is reported to bind to Annexin 1 that enables it to adhere to cell membrane in Annexin 1 positive cells. The two GG domains are composed of seven β-strands and two helices and are located N-terminally after the G8 domain. Each of the four PbH1 domains contain 22 to 23 amino acids and function in polysaccharide hydrolysis. Amino acids sequence 295-591, also known as the B domain, is essential for its retention in the endoplasmic reticulum. CEMIP expression is strongly upregulated in many types of cancer and its higher levels are associated with poor prognosis in cancer patients. Higher expression of CEMIP is reported in ductal carcinoma and invasive breast cancer cells, gastric cancers, and in colon adenocarcinomas. It is considered as a potential blood-borne biomarker for colorectal cancers. The knockdown of CEMIP gene has been shown to significantly reduce metastasis to the lung, liver, and various other organs. (Ref.: Domanegg, K., et al (2022). Cancers (Basel). 14(20); 5093; Fink, SP., et al. (2015). Oncotarget, Vol. 6(31); 30500-30515).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CEMIP, clone PW-3, Cat. No. MABC1764, is a mouse monoclonal antibody that detects CEMIP and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CEMIP in Caco-2 and HT29 cell lysates.

      Immunohistochemistry Applications: A representative lot detected CEMIP, clone PW-3 in Immunohistochemistry applications (Fink, S.P., et al. (2015). Oncotarget. 6(31):30500-15).

      Western Blotting Analysis: A representative lot detected CEMIP in Western Blotting applications (Fink, S.P., et al. (2015). Oncotarget. 6(31):30500-15).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenV5/His-tagged, full-length, recombinant human Cell migration-inducing and hyaluronan-binding protein (CEMIP).
      EpitopeUnknown
      ClonePW-3
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone PW-3 is a mouse monoclonal antibody that detects Cell migration-inducing and hyaluronan-binding protein (CEMIP).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEMIP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight180 kDa observed. 153.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DLD-1 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CEMIP in DLD-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1764-25UG 04065270321824